Examining how post-transcriptional gene regulation mechanisms affect stem cell function

Stem cells are critical biological precursors, which can give rise to a wide variety of different cell types and offer considerable potential in regenerative medicine. This project aims to understand how the post-transcriptional regulation of genes can influence the ability of stem cells to function. This understanding will help us to better manipulate stem cells for therapeutic purposes and to identify markers of stem cell function that could be used in the treatment of chronic diseases.

The project will employ a CRISPR/Cas9 gene editing approach, to develop stem cells models that have impaired post-transcriptional gene control. Both pluripotent and multipoint cell models will be characterised, including embryonic stem cells, induced pluripotent stem cells and adult mesenchyme-derived stem cells.

The project would be suitable for a motivated student who wishes to develop skills in molecular biology, cell culture and transcriptomic data handling. It is an excellent opportunity to perform research using cutting edge technologies in an under-developed area of stem cell biology.

You will be supervised by a team based in Department of Musculoskeletal and Ageing Sciences in the Institute of Life Course and Medical Sciences at the University of Liverpool. The supervisors have wide-ranging expertise and will provide training and support in all relevant laboratory and analysis techniques. Furthermore, all postgraduate students undertake a programme of post graduate researcher development, which aims to enhance their skills for a successful research experience and career.

The Institute of Life Course and Medical Science is fully committed to promoting gender equality in all activities. It aims to foster a supportive working environment and holds a silver Athena SWAN award in recognition of its on-going commitment to this.

Applications should be made to project supervisors in the first instance via CV and cover letter. This is for all applications. Only when a candidate has been selected following interview will a formal online application be required.